免疫共沉淀(Co-Immunoprecipitation)是以抗體和抗原之間的專一性作用為基礎的用于研究蛋白質相互作用的經(jīng)典方法,。是確定兩種蛋白質在完整細胞內生理性相互作用的有效方法,?;驹恚涸诩毎呀庖褐屑尤肟乖禺愋缘目贵w,抗體,、抗原以及與抗原具有相互作用的蛋白質通過抗原與抗體之間的免疫反應形成免疫復合物,洗脫收集復合物,SDS-PAGE電泳,western blot或質譜可驗證或鑒定與抗原互作的蛋白質,。
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