質(zhì)譜法分析蛋白的基本原理是通過電離源將蛋白質(zhì)分子轉(zhuǎn)化為離子,,然后利用質(zhì)譜分析儀的電場,、磁場將具有特定質(zhì)量與電荷比值(M/Z)的蛋白質(zhì)離子分離開來,經(jīng)過離子檢測器收集分離的離子,,確定離子的M/Z值,,分析鑒定未知蛋白。通常結(jié)合相應(yīng)的處理及其他技術(shù),,能夠比較準確,、快速地鑒定蛋白質(zhì)。
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